Protein Molecular Weight Calculator Mass Spectrometry

Consequently for each one the theoretical molecular weight needed to be calculated.

Protein molecular weight calculator mass spectrometry. All trypsin chymotrypsin v8 protease. In general we can determine the weight with an accuracy within 10 ppm 1 da for a 100 kda protein. The analysis is very sensitive. This calculator allows to identify some adduct ions from esi ms electrospray mass spectrometry measurements or other soft ionization techniques like ci ms or fi ms or fd ms or apci ms or maldi tof.

P a peak in the mass spectrum m total mass of an ion z total charge m r average mass of the protein protein mw calculation from esi spectra if p 1 942 753 andp 2 998 067 p m z p 1 m r z 1 z 1 p 2 m r z 1 1 z 1 1 942 753 m r z 1 z 1 942 753z 1 mr z 1 941 753z 1 m r 998 067 941 753 z 1 z 1 1 z 1 1 998 067z 1 997 067 942 753 z 1 1 998 067 942 753 z 1 996 067 55 314z. Alternatively enter a protein sequence in single letter code. You can append copies of commonly used epitopes and fusion proteins using the supplied list. However even for these prominent proteins reliable mass spectrometry reference data do not always exist.

We can perform the analysis on a few µg of protein. This is the php based online version of esiprot. Albu human or uniprot knowledgebase accession numbers ac e g. With our setup you get access to top of the line mass spectrometry instruments that provide very accurate molecular weight mw of both proteins and antibodies.

To generate mass spectrometry sample data six well studied standard proteins between 12 4 and 66 7 kda were chosen. Percent difference maximum difference daltons min max. Esiprot online enables the charge state determination and molecular weight calculation for low resolution electrospray ionization esi mass spectrometry ms data of proteins. Use monoisotopic masses not isotopic averages methionine to selenomethionine cysteine to selenocysteine.

Mass range daltons calculate masses of b and y daughter ions. Please enter one or more uniprotkb swiss prot protein identifiers id e g. Actually this task should be done by every good lc ms software automatically directly integrated no questions asked. The theoretical pi and mw molecular weight will then be computed.

Molecular weight molecular weight scan mass spectrometry result. Protein molecular weight calculator protein molecular weight accepts a protein sequence and calculates the molecular weight.